Chemoselective Bioconjugation of Triazole Phosphonites in Aqueous Media
M.R.J. Vallée, P. Majkut, D. Krause, M. Gerrits, C.P.R. Hackenberger – 2015
Readily accessible and versatile phosphonite building blocks with improved stability against hydrolysis were used for the efficient metal-free functionalization of peptides and proteins in aqueous buffers at low micromolar concentrations. The application of this protocol to the immobilization of a Rasa1-SH2 domain revealed high binding affinity to the human T-cell protein ADAP and supports the applicability of triazole phosphonites for protein modifications without harming their function.