Remote control of lipophilic nucleic acids domain partitioning by DNA hybridization and enzymatic cleavage

M. Schade, A. Knoll, A. Vogel, O. Seitz, J. Liebscher, D. Huster, A. Herrmann, A. Arbuzova – 2012

Lateral partitioning of lipid-modified molecules between liquid-disordered (ld) and liquid-ordered (lo) domains depends on the type of lipid modification, presence of a spacer, membrane composition, and temperature. Here, we show that the lo domain partitioning of the palmitoylated peptide nucleic acid (PNA) can be influenced by formation of a four-component complex with the ld domain partitioning tocopherol-modified DNA: the PNA–DNA complex partitioned into the ld domains. Enzymatic cleavage of the DNA linker led to the disruption of the complex and restored the initial distribution of the lipophilic nucleic acids into the respective domains. This modular system offers strategies for dynamic functionalization of biomimetic surfaces, for example, in nanostructuring and regulation of enzyme catalysis, and it provides a tool to study the molecular basis of controlled reorganization of lipid-modified proteins in membranes, for example, during signal transduction.

Titel
Remote control of lipophilic nucleic acids domain partitioning by DNA hybridization and enzymatic cleavage
Verfasser
M. Schade, A. Knoll, A. Vogel, O. Seitz, J. Liebscher, D. Huster, A. Herrmann, A. Arbuzova
Datum
2012
Kennung
10.1021/ja309256t
Zitierweise
J. Am. Chem. Soc., 2012, 134 (50), 20490–20497
Art
Text
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