Env decorated Phosphatidylserine Liposomes Trigger Phagocytosis of HIV-virus-like Particles in Macrophages.

A. Gramatica, R. A., Petazzi, M. Lehmann, J. Ziomkowska, A. Herrmann, S. Chiantia, – 2014

Macrophages represent an important cellular target of HIV-1. Interestingly, they are also believed to play a potential role counteracting its infection. However, HIV-1 is known to impair macrophage immune functions such as antibody-mediated phagocytosis. Here, we present immunoliposomes that can bind HIV-1 virus-like particles (HIV-VLPs) while being specifically phagocytosed by macrophages, thus allowing the co-internalization of HIV-VLPs. These liposomes are decorated with anti-Env antibodies and contain phosphatidylserine (PS). PS mediates liposome internalization by macrophages via a mechanism not affected by HIV-1. Hence, PS-liposomes mimic apoptotic cells and are internalized into the macrophages due to specific recognition, carrying the previously bound HIV-VLPs. With a combination of flow cytometry, confocal live-cell imaging and electron microscopy we demonstrate that the PS-immunoliposomes presented here are able to elicit efficient HIV-VLPs phagocytosis by macrophages and might represent a new nanotechnological approach to enhance HIV-1 antigen presentation and reduce the ongoing inflammation processes.

Titel
Env decorated Phosphatidylserine Liposomes Trigger Phagocytosis of HIV-virus-like Particles in Macrophages.
Verfasser
A. Gramatica, R. A., Petazzi, M. Lehmann, J. Ziomkowska, A. Herrmann, S. Chiantia,
Datum
2014
Kennung
10.1016/j.nano.2014.02.008
Zitierweise
Nanomedicine, 2014,10 (5), 981-989
Art
Text
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