Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC

F. Erlekam, S. Igde, S. Röblitz, L. Hartmann, M. Weber – 2019

In addition to the conventional Isothermal Titration Calorimetry (ITC), kinetic ITC (kinITC) not only gains thermodynamic information, but also kinetic data from a biochemical binding process. Moreover, kinITC gives insights into reactions consisting of two separate kinetic steps, such as protein folding or sequential binding processes. The ITC method alone cannot deliver kinetic parameters, especially not for multivalent bindings. This paper describes how to solve the problem using kinITC and an invariant subspace projection. The algorithm is tested for multivalent systems with different valencies.

Titel
Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC
Verfasser
F. Erlekam, S. Igde, S. Röblitz, L. Hartmann, M. Weber
Datum
2019
Kennung
10.3390/computation7030046
Zitierweise
Computation 2019, 7(3), 46
Art
Text
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