A crucial role of L-selectin in C protein-induced experimental polymyositis in mice.

K. Oishi, Y. Hamaguchi, T. Matsushita, M. Hasegawa, N. Okiyama, J. Dernedde, M. Weinhart, R. Haag, T.F. Tedder, K. Takehara, H. Kohsaka, M. Fujimoto – 2014

To investigate the role of adhesion molecules in C protein–induced myositis (CIM), a murine model of polymyositis (PM). CIM was induced in wild-type mice, L-selectin–deficient (L-selectin−/−) mice, intercellular adhesion molecule 1 (ICAM-1)–deficient (ICAM-1−/−) mice, and mice deficient in both L-selectin and ICAM-1 (L-selectin−/−ICAM-1−/− mice). Myositis severity, inflammatory cell infiltration, and messenger RNA expression in the inflamed muscles were analyzed. The effect of dendritic polyglycerol sulfate, a synthetic inhibitor that suppresses the function of L-selectin and endothelial P-selectin, was also examined. L-selectin−/− mice and L-selectin−/−ICAM-1−/− mice developed significantly less severe myositis compared to wild-type mice, while ICAM-1 deficiency did not inhibit the development of myositis. L-selectin−/− mice that received wild-type T cells developed myositis. Treatment with dendritic polyglycerol sulfate significantly diminished the severity of myositis in wild-type mice compared to treatment with control. These data indicate that L-selectin plays a major role in the development of CIM, whereas ICAM-1 plays a lesser role, if any, in the development of CIM. L-selectin–targeted therapy may be a candidate for the treatment of PM.

Titel
A crucial role of L-selectin in C protein-induced experimental polymyositis in mice.
Verfasser
K. Oishi, Y. Hamaguchi, T. Matsushita, M. Hasegawa, N. Okiyama, J. Dernedde, M. Weinhart, R. Haag, T.F. Tedder, K. Takehara, H. Kohsaka, M. Fujimoto
Datum
2014
Kennung
10.1002/art.38630
Quelle/n
Zitierweise
Arthritis Rheumatol. 2014, 66, 1864-1871
Art
Text
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