Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids.

Ying Ma, Bala N.S. Thota, Rainer Haag, and Nediljko Budisa – 2015

Polyglycerol dendrimers as an important class of polymeric materials especially attractive for covalent attachment to therapeutic proteins as a useful alternative to traditional PEGylation procedures. Herein, we combine in vivo noncanonical amino acid (ncAA) incorporation and chemoselective conjugation in vitro to produce novel hybrid protein–dendrimer conjugates with the defined architectures. We incorporated Azidohomoalanine (Aha) as methionine substitute in vivo into various protein scaffolds to allow non-invasive dendrimer conjugations (dendronylation). Our approach makes recombinant proteins accessible for the design of multivalent dendrimer conjugates since it enables the preparation of many sequences with various positions for regioselective dendronylation.

Titel
Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids.
Verfasser
Ying Ma, Bala N.S. Thota, Rainer Haag, and Nediljko Budisa
Datum
2015
Kennung
10.1016/j.bmcl.2015.09.055
Zitierweise
Bioorg. Med. Chem. Lett.,2015, 25(22), 5247-5249
Art
Text
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