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Chemoselective Bioconjugation of Triazole Phosphonites in Aqueous Media

M.R.J. Vallée, P. Majkut, D. Krause, M. Gerrits, C.P.R. Hackenberger – 2015

Readily accessible and versatile phosphonite building blocks with improved stability against hydrolysis were used for the efficient metal-free functionalization of peptides and proteins in aqueous buffers at low micromolar concentrations. The application of this protocol to the immobilization of a Rasa1-SH2 domain revealed high binding affinity to the human T-cell protein ADAP and supports the applicability of triazole phosphonites for protein modifications without harming their function.

Titel
Chemoselective Bioconjugation of Triazole Phosphonites in Aqueous Media
Verfasser
M.R.J. Vallée, P. Majkut, D. Krause, M. Gerrits, C.P.R. Hackenberger
Datum
2015
Kennung
10.1002/chem.201404690
Zitierweise
Chem. Eur. J. 2015, 21(3), 970-974
Art
Text
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