Live-cell MRI with Xenon Hyper-CEST Biosensors Targeted to Metabolically Labeled Cell-Surface Glycans

C.Witte, V. Martos, H. M. Rose, S. Reinke, S. Klippel, L. Schröder, C. P. R. Hackenberger – 2015

The targeting of metabolically labeled glycans with conventional MRI contrast agents has proved elusive. In this work, which further expands the utility of xenon Hyper-CEST biosensors in cell experiments, we present the first successful molecular imaging of such glycans using MRI. Xenon Hyper-CEST biosensors are a novel class of MRI contrast agents with very high sensitivity. We designed a multimodal biosensor for both fluorescent and xenon MRI detection that is targeted to metabolically labeled sialic acid through bioorthogonal chemistry. Through the use of a state of the art live-cell bioreactor, it was demonstrated that xenon MRI biosensors can be used to image cell-surface glycans at nanomolar concentrations.

Titel
Live-cell MRI with Xenon Hyper-CEST Biosensors Targeted to Metabolically Labeled Cell-Surface Glycans
Verfasser
C.Witte, V. Martos, H. M. Rose, S. Reinke, S. Klippel, L. Schröder, C. P. R. Hackenberger
Datum
2015
Kennung
10.1002/anie.201410573
Zitierweise
Angew. Chem. Int. Ed., 2015, 54, 2806–2810
Art
Text
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