Chemoselective Staudinger-Phosphite Reaction of Azides for the Phosphorylation of Proteins

R. Serwa, I. Wilkening, G. del Signore, M. Mühlberg, I. Claußnitzer, C. Weise, M. Gerrits, C.P.R. Hackenberger – 2009

Extending the toolbox: The title reaction was identified as a chemoselective means to modify azides in peptides and proteins in high yields at room temperature in various solvents including aqueous buffers at physiological pH. In combination with nonnatural protein translation the Staudinger-phosphite reaction allows the site-specific incorporation of phosphorylated Tyr analogues in proteins.

Titel
Chemoselective Staudinger-Phosphite Reaction of Azides for the Phosphorylation of Proteins
Verfasser
R. Serwa, I. Wilkening, G. del Signore, M. Mühlberg, I. Claußnitzer, C. Weise, M. Gerrits, C.P.R. Hackenberger
Datum
2009
Kennung
10.1002/anie.200902118
Zitierweise
Angew. Chem. Int. Ed. 2009, 47, 8234-8239
Art
Text
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