Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2

A. Pechstein, J. Bacetic, A. Vahedi-Faridi, K. Gromova, A. Sundborger, N. Tomlin, G. Krainer, O. Vorontsova, J.G. Schäfer, S.G. Owe, M.A. Cousin, W. Saenger, O. Shupliakov, V. Haucke – 2010

Clathrin-mediated synaptic vesicle (SV) recycling involves the spatiotemporally controlled assembly of clathrin coat components at phosphatidylinositiol (4, 5)-bisphosphate [PI(4,5)P2]-enriched membrane sites within the periactive zone. Such spatiotemporal control is needed to coordinate SV cargo sorting with clathrin/AP2 recruitment and to restrain membrane fission and synaptojanin-mediated uncoating until membrane deformation and clathrin coat assembly are completed. The molecular events underlying these control mechanisms are unknown. Here we show that the endocytic SH3 domain-containing accessory protein intersectin 1 scaffolds the endocytic process by directly associating with the clathrin adaptor AP2. Acute perturbation of the intersectin 1-AP2 interaction in lamprey synapses in situ inhibits the onset of SV recycling. Structurally, complex formation can be attributed to the direct association of hydrophobic peptides within the intersectin 1 SH3A-B linker region with the “side sites” of the AP2 α- and β-appendage domains. AP2 appendage association of the SH3A-B linker region inhibits binding of the inositol phosphatase synaptojanin 1 to intersectin 1. These data identify the intersectin-AP2 complex as an important regulator of clathrin-mediated SV recycling in synapses.

Titel
Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2
Verfasser
A. Pechstein, J. Bacetic, A. Vahedi-Faridi, K. Gromova, A. Sundborger, N. Tomlin, G. Krainer, O. Vorontsova, J.G. Schäfer, S.G. Owe, M.A. Cousin, W. Saenger, O. Shupliakov, V. Haucke
Datum
2010
Kennung
10.1073/pnas.0911073107
Zitierweise
PNAS 2010, 107(9), 4206-4211
Art
Text
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