Ascorbate as an alternative to thiol additives in native chemical ligation.

H. Rohde, J. Schmalisch, Z. Harpaz, F. Diezmann, O. Seitz – 2011

Vitamin C for peptide ligation: Phosphine and thiol additives maintain reducing environments and increase the reactivities of peptide thioesters in native chemical ligation. Thiol additives such as thiophenol also act as radical scavengers that inhibit phosphine-induced desulfurization of cysteine. This role can be assumed by the odourless, nontoxic, highly water-soluble and inexpensive ascorbate, which can replace the usually added thiols.

Titel
Ascorbate as an alternative to thiol additives in native chemical ligation.
Verfasser
H. Rohde, J. Schmalisch, Z. Harpaz, F. Diezmann, O. Seitz
Datum
2011
Kennung
10.1002/cbic.201100179
Zitierweise
Chem.Bio.Chem. 2011, 12, 1396 - 1400
Art
Text
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